The A985 road will be closed between Longannet Roundabout and Kincardine Bridge on Wednesday night.
It will be shut to traffic from 7.30pm until 6am on Thursday morning to allow BEAR Scotland to repair defects in the carriageway.
A signed diversion route will be in place via the A977 Toll Road and North Approach Road, adding an estimated 1 minute and 0.1 miles to affected journeys.
Access for emergency vehicles will be maintained at all times.
Tommy Deans, BEAR Scotland’s south east network manager, said: “It’s important that we complete these essential repairs quickly and have scheduled the works at night when traffic is lightest.
“The diversion is short, so we don’t expect this to cause any significant disruption. We thank road users and the local community for their patience.”
BEAR Scotland are Transport Scotland’s operating company. All works are weather dependent and subject to postponement in the event of adverse conditions.
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